Bioinformatics Approaches for the Identification and Annotation of RNA Editing Sites

deletion editing (insertion or deletion of nucleotides). With the development of high-throughput next-generation sequencing technologies, increasing numbers of RNA editing sites are rapidly being discovered. Accordingly, many bioinformatics tools and algorithms to identify novel RNA editing sites and semantic annotation of known information are increasingly being introduced. Each method, however, is not free from limitations. For example, numerous false positives are found in the newly discovered sites. 2-4) It is in part due to the limitation of the currently available highthroughput sequencing technologies and in part due to the incompleteness of bioinformatics analysis methods. This review summarizes modern bioinformatics approaches that discover and annotate RNA editing sites. Analytic methods do not work alone solely on RNA sequencing data. This review also includes the strategic steps used in the analysis of RNA editing, related bioinformatics resources that are useful in the analysis of RNA editing and methods to compare the analysis to further improve the analysis results. Bioinformatics Approaches for the Identification and Annotation of RNA Editing Sites